Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.11889/8231
Title: Molecular, antibiogram and serological typing of Staphylococcus aureus isolates recovered from Al-Makased hospital in East Jerusalem
Authors: Essawi, Tamer 
Na’was, Tarek 
Hawwari, Abbass 
Wadi, Suzan 
Doudin, Anwar 
Fattom, Ali Ibrahim 
Keywords: Staphylococcus aureus infections - Treatment - Jerusalem;Nosocomial infection - Treatment - Jerusalem;Cross Infection - Treatment - Jerusalem;Epidemiology - Treatment - Jerusalem;pulsed-field-gel electrophoresis - Jerusalem
Issue Date: 1998
Abstract: Staphylococcus aureus is a major cause of nosocomial infections and a risk in patients who have either undergone surgery or are on haemodialysis. The S. aureus infections in patients admitted to the clinical departments of Al-Makased Charitable Hospital in Jerusalem during a period of one year were investigated. Isolates included were from blood, surgical wounds, or other non superficial sites. Of 63 isolates available for analysis, 46 (73.0%) expressed type 8 capsular polysaccharide; 13 (20.7%), type 5 capsular polysaccharide; only 4 isolates (6.3%) did not express type 5 or type 8 antibodies. The strains fitted in 7 different antibiogram types, with the type showing resistance only to penicillin and ampicillin prevalent in 34 out of 63 isolates (54.0%). Of the 12 methicillin-resistant S. aureus (MRSA) isolates (19.1%), 8(66.7%) possessed the type 8 capsule and 4(33.7%) the type 5 capsule. Pulsed-field gel electrophoresis of all isolates with the restriction endonuclease enzymes Sma I revealed 34 patterns demonstrating that no single methicillin-sensitive S. aureus strain was endemic in the hospital. However, all MRSA isolates with a type 8 capsule showed identical PFGE patterns using the 2 restriction-endonuclease enzymes Sma I and SST II. Moreover, type 5 isolates showed identical patterns (one isolate differed from the rest with one band only). These data suggest and confirm the clonality of type 5 and type 8 MRSA isolates. Analysing the results of the capsular and antibiogram typing schemes in conjunction proved useful and suggested that such an analysis can be employed as a helpful epidemiological tool in hospitals with limited resources.
URI: http://hdl.handle.net/20.500.11889/8231
Appears in Collections:Fulltext Publications

Show full item record

Page view(s)

10
checked on Jun 27, 2024

Download(s)

6
checked on Jun 27, 2024

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.