Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.11889/4497
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dc.contributor.authorAbu Hamdah, Rania-
dc.contributor.authorCho, Won-Jin-
dc.contributor.authorCho, Sang-Joon-
dc.contributor.authorJeremic, Aleksandar-
dc.contributor.authorKelly, Marie L.-
dc.contributor.authorIlie, Alina Elena-
dc.contributor.authorJena, Bhanu P.-
dc.date.accessioned2017-03-11T08:08:29Z-
dc.date.available2017-03-11T08:08:29Z-
dc.date.issued2003-
dc.identifier.urihttp://hdl.handle.net/20.500.11889/4497-
dc.description.abstractAquaporins (AQP) are involved in rapid and active gating of water across biological membranes. The molecular regulation of AQP is unknown. Here we report the isolation, identification and reconstitution of the regulatory complex of AQP-1. AQP-1 and G i3 have been implicated in GTP-induced gating of water in zymogen granules (ZG), the secretory vesicles in exocrine pancreas. In the present study, detergent-solubilized ZGs immunoprecipitated with monoclonal AQP-1 antibody, co-isolates AQP-1, PLA2,G i3, potassium channel IRK-8, and the chloride channel ClC-2. Exposure of ZGs to either the potassium channel blocker glyburide, or the PLA2 inhibitor ONO-RS-082, blocked GTP-induced ZG swelling. RBC known to possess AQP-1 at the plasma membrane, swell on exposure to the G i–agonist mastoparan, and respond similarly to ONO-RS-082 and glyburide, as ZGs. Liposomes reconstituted with the AQP-1 immunoisolated complex from solubilized ZG, also swell in response to GTP. Glyburide or ONO-RS-082 abolished the GTP effect. Immunoisolate-reconstituted planar lipid bilayers demonstrate conductance, which is sensitive to glyburide and an AQP-1 specific antibody. Our results demonstrate a G i3-PLA2 mediated pathway and potassium channel involvement in AQP-1 regulation.en_US
dc.language.isoen_USen_US
dc.publisherCell Biology International 28 (2004) 7–17en_US
dc.subjectBiological transporten_US
dc.subjectMolecular biologyen_US
dc.subjectAquaporinsen_US
dc.subjectAtomic force microscopyen_US
dc.subjectElectrophysiologyen_US
dc.subjectLight - Scatteringen_US
dc.titleRegulation of the water channel aquaporin-1 : isolation and reconstitution of the regulatory complexen_US
dc.typeArticleen_US
newfileds.departmentPharmacy - Nursing and Health Professionsen_US
newfileds.item-access-typeopen_accessen_US
newfileds.thesis-prognoneen_US
newfileds.general-subjectnoneen_US
item.fulltextWith Fulltext-
item.grantfulltextopen-
item.languageiso639-1other-
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