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Title: Structure and composition of the fusion pore
Authors: Jena, Bhanu P.
Cho, Sang-Joon
Jeremic, Aleksandar
Stromer, Marvin H.
Abu Hamdah, Rania
Keywords: Atomic force microscopy;Cell membranes;Cell hybridization
Issue Date: 2003
Publisher: Biophysical Journal Volume 84 February 2003 1337–1343
Abstract: Earlier studies using atomic force microscopy (AFM) demonstrated the presence of fusion pores at the cell plasma membrane in a number of live secretory cells, revealing their morphology and dynamics at nm resolution and in real time. Fusion pores were stable structures at the cell plasma membrane where secretory vesicles dock and fuse to release vesicular contents. In the present study, transmission electron microscopy confirms the presence of fusion pores and reveals their detailed structure and association with membrane-bound secretory vesicles in pancreatic acinar cells. Immunochemical studies demonstrated that t-SNAREs, NSF, actin, vimentin, a-fodrin and the calcium channels a1c and b3 are associated with the fusion complex. The localization and possible arrangement of SNAREs at the fusion pore are further demonstrated from combined AFM, immunoAFM, and electrophysiological measurements. These studies reveal the fusion pore or porosome to be a cup-shaped lipoprotein structure, the base of which has t-SNAREs and allows for docking and release of secretory products from membrane-bound vesicles.
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