Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.11889/4175
Title: Exaggerated inflammatory responses mediated by burkholderia cenocepacia in human macrophages derived from cystic fibrosis
Authors: Abu Khweek, Arwa
Keywords: Cystic fibrosis
Ralstonia solanacearum
Issue Date: 2012
Abstract: Cystic Fibrosis (CF) is accompanied with heightened inflammation worsened by drug resistant Burkholderia cenocepacia. Human CF macrophage responses to B. cenocepacia are poorly characterized and variable in the literature. Therefore, we examined human macrophage responses to the epidemic B. cenocepacia J2315 strain in order to identify novel anti-inflammatory targets. Peripheral blood monocyte derived macrophages were obtained from 23 CF and 27 non-CF donors. Macrophages were infected with B. cenocepacia J2315 and analyzed for cytokines, cytotoxicity, and microscopy. CF macrophages demonstrated significant increases in IL-1β, IL-10, MCP-1, and IFN-γ production in comparison to non-CF controls. CF patients on prednisone exhibited globally diminished cytokines compared to controls and other CF patients. CF macrophages also displayed increased bacterial burden and cell death. In conclusion, CF macrophages demonstrate exaggerated IL-1β, IL-10, MCP-1, and IFN-γ production and cell death during B. cenocepacia infection. Treatment with corticosteroids acutely suppressed cytokine responses
Description: Authors include Benjamin T. Kopp, Basant A. Abdulrahman, Surender B. Kumar, Anwari Akhter, Richard Montione, Mia F. Tazi, Kyle Caution, Karen McCoy, and Amal O. Amer
URI: http://hdl.handle.net/20.500.11889/4175
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