Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.11889/2601
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dc.contributor.authorStiban, Johnny
dc.contributor.authorPrieto, Manuel
dc.contributor.authorFuterman, Tony
dc.contributor.authorSilva, Liana
dc.date.accessioned2016-10-13T09:34:08Z
dc.date.available2016-10-13T09:34:08Z
dc.date.issued2014
dc.identifier.urihttp://hdl.handle.net/20.500.11889/2601
dc.descriptionJournal of Lipid Research, Volume 55, 2014en_US
dc.description.abstractCeramide (Cer) is involved in the regulation of several cellular processes by mechanisms that depend on Cer-induced changes on membrane biophysical properties. Accumulating evidence shows that Cers with different N - acyl chain composition differentially impact cell physiology, which may in part be due to specific alterations in membrane biophysical properties. We now address how the sphingolipid (SL) N-acyl chain affects membrane properties in cultured human embryonic kidney cells by over expressing different Cer synthases (CerSs). Our results show an increase in the order of cellular membranes in CerS2-transfected cells caused by the enrichment in very long acyl chain SLs. Formation of Cer upon treatment of cells with bacterial sphingomyelinase promoted sequential changes in the properties of the membranes: after an initial increase in the order of the fluid plasma membrane, reorganization into domains with gel-like properties whose characteristics are dependent on the acyl chain structure of the Cer was observed. Moreover, the extent of alterations of membrane properties correlates with the amount of Cer formed. These data reinforce the significance of Cer-induced changes on membrane biophysical properties as a likely molecular mechanism by which different acyl chain Cers exert their specific biological actions.—Pinto, S. N., E. L. Laviad, J. Stiban, S. L. Kelly, A. H. Merrill, Jr., M. Prieto, A. H. Futerman, and L. C. Silva. Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chain. J. Lipid Res. 2014. 55: 53–61
dc.language.isoenen_US
dc.publisherResearchGateen_US
dc.subject.lcshMembranes (Biology)
dc.subject.lcshSphingolipids - Physiological effect
dc.subject.lcshBiological transport
dc.subject.lcshMembrane lipids
dc.subject.lcshMembranes (Biology) - Mechanical properties
dc.titleChanges in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chainen_US
dc.typeArticleen_US
newfileds.departmentDepartment of Biology and Biochemistryen_US
newfileds.item-access-typeopen_accessen_US
item.languageiso639-1other-
item.fulltextWith Fulltext-
item.grantfulltextopen-
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