Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.11889/7927
Title: Stimulated Release of Exogenous GABA and Glutamate from Cerebral Cortical Synaptosomes and Brain Slices by Bis(acetato)tetrakis(imidazole) Copper(II) Complex
Authors: Abdul-Ghani, Abdul-Salam 
Abu-Hijleh, Abdul-Latif 
Qazzaz, Munir 
Muhaisen, Ashraf 
Keywords: Copper(II) complexes;GABA release;Glutamic acid;Glutamate;Brain slices;Synaptosomes;Tetrodotoxin
Issue Date: 2005
Abstract: In these experiments we have tested the effect of bis(acetato)tetrakis (imidazole) copper(II) on the release and uptake of 14C-GABA and 3H-glutamate from brain slices and brain cortical synaptosomes. Cu(OAc)2(Im)4 in concentrations ranging from 1 to 100 µM has increased the release of GABA and glutamate from brain slices and synaptosomal preparations in a dose-related manner when the effect on GABA release is two-fold greater than glutamate and 10-fold greater than alanine. Pretreatment with a GABA uptake inhibitor such as 1–2 mM nipecotic acid has no effect on 14C-GABA release, whereas hydroxy aspartate, the glutamate uptake inhibitor, has elevated the stimulated release of glutamate. Copper(II) chloride, the inorganic form of copper, had no significant effect either on GABA release or on glutamate release. The stimulated release of exogenous GABA and glutamate was Ca2+-dependent, because it was inhibited by EGTA, and neuronal, because it was blocked by tetrodotoxin. The recent results can explain the anticonvulsant activity of Cu(OAc)2(Im)4 against strychnine-induced seizures by increasing the net release of GABA from cortical neurons.
URI: http://hdl.handle.net/20.500.11889/7927
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