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Title: Isolation, identification and molecular characterization of Brucella strains isolated from small ruminants in the West Bank, Palestine
Authors: Awwad, Elena 
Farraj, Mohammad 
Adwan, Kamel M. 
Essawi, Tamer 
Keywords: Brucellosis - Palestine;Small ruminants - Palestine;Serological test - Palestine;B. melitensis - Palestine;Bacterial isolation - Palestine;PCR assay - Palestine;Phylogenic analysis - Palestine
Issue Date: 2011
Publisher: Revue de Medecine Veterinaire
Abstract: The seroprevalence of brucellosis in the small ruminant (sheep and goat) population from infected flocks was investigated in the West Bank (Palestine). 36.8% (129/350) and 32.3% (113/350) of animals were found to be positive by the Rose Bengal test (RBT) and the complement fixation test (CFT) respectively, the agreement score between the 2 methods being 89.1%. The presence of Brucella directly assessed by bacterial isolation and PCR assay in milk samples from 80 seropositive animals was confirmed in 47.5% (38/80) and in 77.5% (62/80) of samples, leading to a moderate agreement score between the 2 methods (70.0%). The phylogenic analysis of a putative open reading frame (ORF) in the haemagglutinin gene revealed that the current B. melitensis isolates were genetically close to those from different Mediterranean countries (France, Spain and Cyprus) and particularly to the Israel strain. This first report of DNA sequencing of Brucella strains in small ruminants in Palestine would greatly help to implement control and preventive measures for brucellosis.
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