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Title: Time-related changes in hepatic and colonic mitochondrial oxygen consumption after abdominal infection in rats
Authors: Herminghaus, Anna 
Papenbrock, Henrike 
Eberhardt, Rebecca 
Vollmer, Christian 
Truse, Richard 
Schulz, Jan 
Bauer, Inge 
Weidinger, Adelheid 
Kozlov, Andrey V. 
Stiban, Johnny 
Picker, Olaf 
Issue Date: 8-Apr-2019
Publisher: Intensive Care Medicine Experimental
Series/Report no.: ;doi: 10.1186/s40635-018-0219-9.
Abstract: BACKGROUND: Evidence suggests that early adaptive responses of hepatic mitochondria occur in experimentally induced sepsis. Little is known about both colonic mitochondrial function during abdominal infection and long-term changes in mitochondrial function under inflammatory conditions. We hypothesize that hepatic and colonic mitochondrial oxygen consumption changes time-dependently after sterile laparotomy and in the course of abdominal infection. The aim of the present study was to investigate the hepatic and colonic mitochondrial respiration after sterile laparotomy and abdominal infection over up to 96 h. METHODS: After approval of the local Animal Care and Use Committee, 95 Wistar rats were randomized into 8 groups (n = 11-12): 1-4 sham (laparotomy only) and 5-8 colon ascendens stent peritonitis (CASP). Healthy, unoperated animals served as controls (n = 9). The mitochondrial respiration in colon and liver homogenates was assessed 24, 48, 72, and 96 h after surgery. Mitochondrial oxygen consumption was determined using a Clark-type electrode. State 2 (oxygen consumption in the presence of the substrates for complexes I and II) and state 3 respiration (ADP dependent) were assessed. The respiratory control ratio (RCR state 3/state 2) and ADP/O ratio (ADP added/oxygen consumed) were calculated for both complexes. Data are presented as means ± SD, two-way ANOVA followed by Tukey's post hoc test. RESULTS: Hepatic RCR was initially (after 24 h) elevated in both operated groups; after 48 h only, the septic group was elevated compared to controls. In CASP groups, the hepatic ADP/O ratio for complex I was elevated after 24 h (vs. controls) and after 48 h (vs. sham) but declined after 72 h (vs. controls). The ADP/O ratio for complex II stayed unchanged over the time period until 96 h. The colonic RCR and ADP/O did not change over time after sham or CASP operation. CONCLUSION: Hepatic, but not colonic, mitochondrial respiration is increased in the initial phase (until 48 h) and normalizes in the longer course of time (until 96 h) of abdominal infection.
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