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Title: Computational and biochemical characterization of two partially overlapping interfaces and multiple weak-affinity K-Ras dimer
Authors: Prakash, Priyanka
Sayyed-Ahmad, Abdallah
Cho, Kwang-Jin
Dolino, Drew M.
Chen, Wei
Li, Hongyang
Grant, Barry J.
Hancock, John F.
Gorfe, Alemayehu A.
Keywords: Membrane proteins;K-Ras dimers;Proteins - Stability;Protein folding
Issue Date: Jan-2017
Source: Prakash, P., Sayyed-Ahmad, A., Cho, K.J., Dolino, D.M., Chen, W., Li, H., Grant, B.J., Hancock, J.F. and Gorfe, A.A., 2017. Computational and biochemical characterization of two partially overlapping interfaces and multiple weak-affinity K-Ras dimers. Scientific Reports, 7, p.40109.
Abstract: Recent studies found that membrane-bound K-Ras dimers are important for biological function. However, the structure and thermodynamic stability of these complexes remained unknown because they are hard to probe by conventional approaches. Combining data from a wide range of computational and experimental approaches, here we describe the structure, dynamics, energetics and mechanism of assembly of multiple K-Ras dimers. Utilizing a range of techniques for the detection of reactive surfaces, protein-protein docking and molecular simulations, we found that two largely polar and partially overlapping surfaces underlie the formation of multiple K-Ras dimers. For validation we used mutagenesis, electron microscopy and biochemical assays under non-denaturing conditions. We show that partial disruption of a predicted interface through charge reversal mutation of apposed residues reduces oligomerization while introduction of cysteines at these positions enhanced dimerization likely through the formation of an intermolecular disulfide bond. Free energy calculations indicated that K-Ras dimerization involves direct but weak protein-protein interactions in solution, consistent with the notion that dimerization is facilitated by membrane binding. Taken together, our atomically detailed analyses provide unique mechanistic insights into K-Ras dimer formation and membrane organization as well as the conformational fluctuations and equilibrium thermodynamics underlying these processes.
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